Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Cell
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (186)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aharoni, A.
Right arrow Articles by O'Connell, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aharoni, A.
Right arrow Articles by O'Connell, A. P.
Agricola
Right arrow Articles by Aharoni, A.
Right arrow Articles by O'Connell, A. P.
Plant Cell, Vol. 12, 647-662, May 2000, Copyright © 2000, American Society of Plant Physiologists

Identification of the SAAT Gene Involved in Strawberry Flavor Biogenesis by Use of DNA Microarrays

Asaph Aharonia, Leopold C. P. Keizerb, Harro J. Bouwmeestera, Zhongkui Suna, Mayte Alvarez-Huertaa, Harrie A. Verhoevena, Jan Blaasa, Adèle M. M. L. van Houwelingena, Ric C. H. De Vosa, Hilko van der Voetb, Ritsert C. Jansenb, Monique Guisa, Jos Molc, Ronald W. Davisd, Mark Schenad, Arjen J. van Tunena, and Ann P. O'Connella
a Business Unit Cell Cybernetics, Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands
b Business Unit Biometry, Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands
c Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
d Department of Biochemistry, Beckman Center, Stanford University Medical Center, Stanford, California 94305

Correspondence to: Asaph Aharoni, a.aharoni{at}plant.wag-ur.nl (E-mail), 31-0317-418094 (fax)

Fruit flavor is a result of a complex mixture of numerous compounds. The formation of these compounds is closely correlated with the metabolic changes occurring during fruit maturation. Here, we describe the use of DNA microarrays and appropriate statistical analyses to dissect a complex developmental process. In doing so, we have identified a novel strawberry alcohol acyltransferase (SAAT) gene that plays a crucial role in flavor biogenesis in ripening fruit. Volatile esters are quantitatively and qualitatively the most important compounds providing fruity odors. Biochemical evidence for involvement of the SAAT gene in formation of fruity esters is provided by characterizing the recombinant protein expressed in Escherichia coli. The SAAT enzyme showed maximum activity with aliphatic medium-chain alcohols, whose corresponding esters are major components of strawberry volatiles. The enzyme was capable of utilizing short- and medium-chain, branched, and aromatic acyl-CoA molecules as cosubstrates. The results suggest that the formation of volatile esters in fruit is subject to the availability of acyl-CoA molecules and alcohol substrates and is dictated by the temporal expression pattern of the SAAT gene(s) and substrate specificity of the SAAT enzyme(s).


Related articles in Plant Cell:

Microarrays: Determining the Balance of Cellular Transcription
Stacey L. Harmer and Steve A. Kay
Plant Cell 2000 12: 613-616. [Full Text]  



This article has been cited by other articles:


Home page
 Plant Physiol.Home page
V. Shulaev, S. S. Korban, B. Sosinski, A. G. Abbott, H. S. Aldwinckle, K. M. Folta, A. Iezzoni, D. Main, P. Arus, A. M. Dandekar, et al.
Multiple Models for Rosaceae Genomics
Plant Physiology, July 1, 2008; 147(3): 985 - 1003.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
I. Hernandez, D. Molenaar, J. Beekwilder, H. Bouwmeester, and J. E. T. van Hylckama Vlieg
Expression of Plant Flavor Genes in Lactococcus lactis
Appl. Envir. Microbiol., March 1, 2007; 73(5): 1544 - 1552.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. M. Brady, T. A. Long, and P. N. Benfey
Unraveling the dynamic transcriptome.
PLANT CELL, September 1, 2006; 18(9): 2101 - 2111.
[Full Text] [PDF]

Home page
 J Exp BotHome page
S. Lunkenbein, E. M. J. Salentijn, H. A. Coiner, M. J. Boone, F. A. Krens, and W. Schwab
Up- and down-regulation of Fragariaxananassa O-methyltransferase: impacts on furanone and phenylpropanoid metabolism
J. Exp. Bot., July 1, 2006; 57(10): 2445 - 2453.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. D. Newcomb, R. N. Crowhurst, A. P. Gleave, E. H.A. Rikkerink, A. C. Allan, L. L. Beuning, J. H. Bowen, E. Gera, K. R. Jamieson, B. J. Janssen, et al.
Analyses of Expressed Sequence Tags from Apple
Plant Physiology, May 1, 2006; 141(1): 147 - 166.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Raab, J. A. Lopez-Raez, D. Klein, J. L. Caballero, E. Moyano, W. Schwab, and J. Munoz-Blanco
FaQR, Required for the Biosynthesis of the Strawberry Flavor Compound 4-Hydroxy-2,5-Dimethyl-3(2H)-Furanone, Encodes an Enone Oxidoreductase
PLANT CELL, April 1, 2006; 18(4): 1023 - 1037.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. M. Tieman, M. Zeigler, E. A. Schmelz, M. G. Taylor, P. Bliss, M. Kirst, and H. J. Klee
Identification of loci affecting flavour volatile emissions in tomato fruits
J. Exp. Bot., March 1, 2006; 57(4): 887 - 896.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Lunkenbein, M. Bellido, A. Aharoni, E. M.J. Salentijn, R. Kaldenhoff, H. A. Coiner, J. Munoz-Blanco, and W. Schwab
Cinnamate Metabolism in Ripening Fruit. Characterization of a UDP-Glucose:Cinnamate Glucosyltransferase from Strawberry
Plant Physiology, March 1, 2006; 140(3): 1047 - 1058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Y. Hirai, M. Klein, Y. Fujikawa, M. Yano, D. B. Goodenowe, Y. Yamazaki, S. Kanaya, Y. Nakamura, M. Kitayama, H. Suzuki, et al.
Elucidation of Gene-to-Gene and Metabolite-to-Gene Networks in Arabidopsis by Integration of Metabolomics and Transcriptomics
J. Biol. Chem., July 8, 2005; 280(27): 25590 - 25595.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
D. Umeno, A. V. Tobias, and F. H. Arnold
Diversifying Carotenoid Biosynthetic Pathways by Directed Evolution
Microbiol. Mol. Biol. Rev., March 1, 2005; 69(1): 51 - 78.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Aharoni, A. P. Giri, F. W.A. Verstappen, C. M. Bertea, R. Sevenier, Z. Sun, M. A. Jongsma, W. Schwab, and H. J. Bouwmeester
Gain and Loss of Fruit Flavor Compounds Produced by Wild and Cultivated Strawberry Species
PLANT CELL, November 1, 2004; 16(11): 3110 - 3131.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Causse, P. Duffe, M. C. Gomez, M. Buret, R. Damidaux, D. Zamir, A. Gur, C. Chevalier, M. Lemaire-Chamley, and C. Rothan
A genetic map of candidate genes and QTLs involved in tomato fruit size and composition
J. Exp. Bot., August 1, 2004; 55(403): 1671 - 1685.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Beekwilder, M. Alvarez-Huerta, E. Neef, F. W.A. Verstappen, H. J. Bouwmeester, and A. Aharoni
Functional Characterization of Enzymes Forming Volatile Esters from Strawberry and Banana
Plant Physiology, August 1, 2004; 135(4): 1865 - 1878.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Dudareva, E. Pichersky, and J. Gershenzon
Biochemistry of Plant Volatiles
Plant Physiology, August 1, 2004; 135(4): 1893 - 1902.
[Full Text] [PDF]

Home page
 Plant CellHome page
J. J. Giovannoni
Genetic Regulation of Fruit Development and Ripening
PLANT CELL, June 1, 2004; 16(suppl_1): S170 - S180.
[Full Text] [PDF]

Home page
 Plant CellHome page
A. Aharoni, A. P. Giri, S. Deuerlein, F. Griepink, W.-J. de Kogel, F. W. A. Verstappen, H. A. Verhoeven, M. A. Jongsma, W. Schwab, and H. J. Bouwmeester
Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants
PLANT CELL, December 1, 2003; 15(12): 2866 - 2884.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
K. Dobbin, J. H. Shih, and R. Simon
Questions and Answers on Design of Dual-Label Microarrays for Identifying Differentially Expressed Genes
J Natl Cancer Inst, September 17, 2003; 95(18): 1362 - 1369.
[Full Text] [PDF]

Home page
 J Exp BotHome page
L. Trainotti, D. Zanin, and G. Casadoro
A cell wall-oriented genomic approach reveals a new and unexpected complexity of the softening in peaches
J. Exp. Bot., August 1, 2003; 54(389): 1821 - 1832.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Goossens, S. T. Hakkinen, I. Laakso, T. Seppanen-Laakso, S. Biondi, V. De Sutter, F. Lammertyn, A. M. Nuutila, H. Soderlund, M. Zabeau, et al.
A functional genomics approach toward the understanding of secondary metabolism in plant cells
PNAS, July 8, 2003; 100(14): 8595 - 8600.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Shalit, I. Guterman, H. Volpin, E. Bar, T. Tamari, N. Menda, Z. Adam, D. Zamir, A. Vainstein, D. Weiss, et al.
Volatile Ester Formation in Roses. Identification of an Acetyl-Coenzyme A. Geraniol/Citronellol Acetyltransferase in Developing Rose Petals
Plant Physiology, April 1, 2003; 131(4): 1868 - 1876.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Alexander and D. Grierson
Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening
J. Exp. Bot., October 1, 2002; 53(377): 2039 - 2055.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Aharoni and A. P. O'Connell
Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays
J. Exp. Bot., October 1, 2002; 53(377): 2073 - 2087.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Moseyko, T. Zhu, H.-S. Chang, X. Wang, and L. J. Feldman
Transcription Profiling of the Early Gravitropic Response in Arabidopsis Using High-Density Oligonucleotide Probe Microarrays
Plant Physiology, October 1, 2002; 130(2): 720 - 728.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. C. D'Auria, F. Chen, and E. Pichersky
Characterization of an Acyltransferase Capable of Synthesizing Benzylbenzoate and Other Volatile Esters in Flowers and Damaged Leaves of Clarkia breweri
Plant Physiology, September 1, 2002; 130(1): 466 - 476.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Hall, M. Beale, O. Fiehn, N. Hardy, L. Sumner, and R. Bino
Plant Metabolomics: The Missing Link in Functional Genomics Strategies
PLANT CELL, July 1, 2002; 14(7): 1437 - 1440.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Aharoni, L. C.P. Keizer, H. C. Van Den Broeck, R. Blanco-Portales, J. Munoz-Blanco, G. Bois, P. Smit, R. C.H. De Vos, and A. P. O'Connell
Novel Insight into Vascular, Stress, and Auxin-Dependent and -Independent Gene Expression Programs in Strawberry, a Non-Climacteric Fruit
Plant Physiology, July 1, 2002; 129(3): 1019 - 1031.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Flores, F. El Yahyaoui, G. de Billerbeck, F. Romojaro, A. Latche, M. Bouzayen, J.-C. Pech, and C. Ambid
Role of ethylene in the biosynthetic pathway of aliphatic ester aroma volatiles in Charentais Cantaloupe melons
J. Exp. Bot., February 1, 2002; 53(367): 201 - 206.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Ivashuta, K. Uchiyama, M. Gau, and Y. Shimamoto
Linear amplification coupled with controlled extension as a means of probe amplification in a cDNA array and gene expression analysis during cold acclimation in alfalfa (Medicago sativa L.)
J. Exp. Bot., February 1, 2002; 53(367): 351 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Crit. Rev. Oral Biol. Med.Home page
K. Iida and I. Nishimura
GENE EXPRESSION PROFILING BY DNA MICROARRAY TECHNOLOGY
Crit. Rev. Oral. Biol. Med., January 1, 2002; 13(1): 35 - 50.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Vainstein, E. Lewinsohn, E. Pichersky, and D. Weiss
Floral Fragrance. New Inroads into an Old Commodity
Plant Physiology, December 1, 2001; 127(4): 1383 - 1389.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Hertzberg, H. Aspeborg, J. Schrader, A. Andersson, R. Erlandsson, K. Blomqvist, R. Bhalerao, M. Uhlen, T. T. Teeri, J. Lundeberg, et al.
A transcriptional roadmap to wood formation
PNAS, November 20, 2001; (2001) 261293398.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
U. Roessner, L. Willmitzer, and A. R. Fernie
High-Resolution Metabolic Phenotyping of Genetically and Environmentally Diverse Potato Tuber Systems. Identification of Phenocopies
Plant Physiology, November 1, 2001; 127(3): 749 - 764.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
U. Roessner, A. Luedemann, D. Brust, O. Fiehn, T. Linke, L. Willmitzer, and A. R. Fernie
Metabolic Profiling Allows Comprehensive Phenotyping of Genetically or Environmentally Modified Plant Systems
PLANT CELL, January 1, 2001; 13(1): 11 - 29.
[Abstract] [Full Text]

Home page
 Plant CellHome page
M. Seki, M. Narusaka, H. Abe, M. Kasuga, K. Yamaguchi-Shinozaki, P. Carninci, Y. Hayashizaki, and K. Shinozaki
Monitoring the Expression Pattern of 1300 Arabidopsis Genes under Drought and Cold Stresses by Using a Full-Length cDNA Microarray
PLANT CELL, January 1, 2001; 13(1): 61 - 72.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
T. Zhu and X. Wang
Large-Scale Profiling of the Arabidopsis Transcriptome
Plant Physiology, December 1, 2000; 124(4): 1472 - 1476.
[Full Text]

Home page
 Plant CellHome page
S. L. Harmer and S. A. Kay
Microarrays: Determining the Balance of Cellular Transcription
PLANT CELL, May 1, 2000; 12(5): 613 - 616.
[Full Text]

Home page
 J. Biol. Chem.Home page
H. Suzuki, T. Nakayama, K. Yonekura-Sakakibara, Y. Fukui, N. Nakamura, M. Nakao, Y. Tanaka, M.-a. Yamaguchi, T. Kusumi, and T. Nishino
Malonyl-CoA:Anthocyanin 5-O-Glucoside-6'''-O-Malonyltransferase from Scarlet Sage (Salvia splendens) Flowers. ENZYME PURIFICATION, GENE CLONING, EXPRESSION, AND CHARACTERIZATION
J. Biol. Chem., December 21, 2001; 276(52): 49013 - 49019.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Hertzberg, H. Aspeborg, J. Schrader, A. Andersson, R. Erlandsson, K. Blomqvist, R. Bhalerao, M. Uhlen, T. T. Teeri, J. Lundeberg, et al.
A transcriptional roadmap to wood formation
PNAS, December 4, 2001; 98(25): 14732 - 14737.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2000 by the American Society of Plant Biologists