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THE PLANT CELL, Vol 1, Issue 5 515-522, Copyright © 1989 by American Society of Plant Biologists
Mutations in the Tobacco Mosaic Virus 30-kD Protein Gene Overcome Tm-2 Resistance in Tomato
T. Meshi, F. Motoyoshi, T. Maeda, S. Yoshiwoka, H. Watanabe and Y. Okada
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan
A resistance-breaking strain of tobacco mosaic virus (TMV), Ltb1, is able
to multiply in tomatoes with the Tm-2 gene, unlike its parent strain, L.
Nucleotide sequence analysis of Ltb1 RNA revealed two amino acid changes in
the 30-kD protein: from Cys68 to Phe and from Glu133 to Lys (from L to
Ltb1). Strains with these two changes generated in vitro multiplied in
tomatoes with the Tm-2 gene and induced essentially the same symptoms as
those caused by Ltb1. Strains with either one of the two changes did not
overcome the resistance as efficiently as Ltb1, although increased levels
of multiplication were observed compared with the L strain. Results showed
that both mutations are involved in the resistance-breaking property of
Ltb1. Sequence analysis indicated that another resistance-breaking strain
and its parent strain had two amino acid changes in the 30-kD protein: from
Glu52 to Lys and from Glu133 to Lys. The fact that the amino acid changes
occurred in or near the well conserved regions in the 30-kD protein
suggests that the mechanism of Tm-2 resistance may be closely related to
the fundamental function of the 30-kD protein, presumably in cell-to-cell
movement.
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