THE PLANT CELL, Vol 1, Issue 4 471-478, Copyright © 1989 by American Society of Plant Biologists
The Pea rbcS-3A Promoter Mediates Light Responsiveness but not Organ Specificity
C. Kuhlemeier, G. Strittmatter, K. Ward and N. H. Chua
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399
The expression of the pea rbcS-3A gene is responsive to light and
restricted to chloroplast-containing cells. Previously, we identified
transcriptional elements between -410 and -50 relative to the transcription
start site as being sufficient for this regulated expression. Here we
investigate the role of the promoter region (-50 to +15) in rbcS-3A
expression. We demonstrate an interaction between the promoter and upstream
sequences located between -189 and -166, which is important for high level
expression. In addition we show that, when the rbcS promoter is driven by a
weak test enhancer, it can confer light-responsive but not organ-specific
expression upon a bacterial reporter gene.