Plant Cell Hybrigenics The Protein Interactions Experts
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THE PLANT CELL, Vol 1, Issue 4 471-478, Copyright © 1989 by American Society of Plant Biologists


RESEARCH ARTICLES

The Pea rbcS-3A Promoter Mediates Light Responsiveness but not Organ Specificity

C. Kuhlemeier, G. Strittmatter, K. Ward and N. H. Chua
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399

The expression of the pea rbcS-3A gene is responsive to light and restricted to chloroplast-containing cells. Previously, we identified transcriptional elements between -410 and -50 relative to the transcription start site as being sufficient for this regulated expression. Here we investigate the role of the promoter region (-50 to +15) in rbcS-3A expression. We demonstrate an interaction between the promoter and upstream sequences located between -189 and -166, which is important for high level expression. In addition we show that, when the rbcS promoter is driven by a weak test enhancer, it can confer light-responsive but not organ-specific expression upon a bacterial reporter gene.


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Copyright © 1989 by the American Society of Plant Biologists