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THE PLANT CELL, Vol 1, Issue 4 427-435, Copyright © 1989 by American Society of Plant Biologists
A Frameshift Mutation Prevents Kunitz Trypsin Inhibitor mRNA Accumulation in Soybean Embryos
K. D. Jofuku, R. D. Schipper and R. B. Goldberg
Department of Biology, University of California, Los Angeles, Los Angeles, California 90024-1606
We investigated the molecular basis of a soybean Kunitz trypsin inhibitor
(KTi) gene mutation that prevents the accumulation of Kunitz trypsin
inhibitor protein during seed development. We found that mRNA encoding the
major Kunitz trypsin inhibitor protein (KTi3 mRNA) is reduced at least
100-fold in null (KTi-) embryos but that KTi3 gene transcriptional activity
is similar in Kunitz trypsin inhibitor producing embryos (KTi+) and in KTi-
embryos. We sequenced the Kunitz trypsin inhibitor KTi3 gene from both
KTi3+ and KTi3- lines and found that these genes differ by only three
nucleotides (+481, +486, and +487) within the KTi3 coding region.
Alteration of these nucleotides results in a frameshift within the KTi3-
gene that causes premature termination during translation. Our results
suggest that the Kti3- frameshift mutation results in KTi3- mRNA
destabilization and leads to a drastic reduction in KTi3 mRNA prevalence.
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